WNK1 antibody (Internal Region)

Catalog No. ABIN213554

The Rabbit Polyclonal anti-WNK1 antibody has been validated for ELISA, IHC and IHC (p). It is suitable to detect WNK1 in samples from Human, Mouse, Rat, Horse, Monkey, Rabbit, Pig, Bat, Hamster, Chicken and Cow.

Quick Overview for WNK1 antibody (Internal Region) (ABIN213554)

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Reactivity: Human, Mouse, Rat, Horse, Monkey, Rabbit, Pig, Bat, Hamster, Chicken, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNK1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WNK1 Antibody

Binding Specificity: Internal Region

Brand: IHC-plus™

Specificity: Human WNK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%) Platypus, Xenopus (95%) Lizard (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from internal region of human WNK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%), Platypus, Xenopus (95%), Lizard (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (7 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for WNK1

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Alternative Name: WNK1 / p65

Background: Name/Gene ID: WNK1
Subfamily: WNK
Family: Protein Kinase

Synonyms: WNK1, Erythrocyte 65 kDa protein, HSAN2, KIAA0344, Kinase deficient protein, HWNK1, PHA2C, PRKWNK1, PSK, HSN2, KDP

Gene ID: 65125