ATIC antibody

Catalog No. ABIN214223

The Mouse Monoclonal anti-ATIC antibody is suitable to detect ATIC in samples from Human, Dog, Rabbit, Horse, Cow, Pig, Monkey, Drosophila melanogaster and Xenopus laevis. It has been validated for WB, IHC and IHC (p).

Quick Overview for ATIC antibody (ABIN214223)

Target: ATIC (5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC))

Reactivity: Human, Dog, Rabbit, Horse, Cow, Pig, Monkey, Drosophila melanogaster, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATIC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: F38 P7 H9

Product Details anti-ATIC Antibody

Specificity: ATIC

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse, Rabbit, Pig, Xenopus, Drosophila, Beetle (100%) Mouse, Rat, Hamster, Bat, Opossum, Turkey, Chicken, Platypus, Stickleback, Mosquito (90%) Elephant, Salmon, Zebrafish, Seq squirt, Sea anemone, Ant, Nematode (80%).

Purification: Affinity purified

Immunogen: Synthetic peptide from human ATIC. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse, Rabbit, Pig, Xenopus, Drosophila, Beetle (100%), Mouse, Rat, Hamster, Bat, Opossum, Turkey, Chicken, Platypus, Stickleback, Mosquito (90%), Elephant, Salmon, Zebrafish, Seq squirt, Sea anemone, Ant, Nematode (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (15 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide at 0.5 mg/mL. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for ATIC

Target: ATIC (5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC))

Alternative Name: AICAR / ATIC

Background: Name/Gene ID: ATIC

Synonyms: ATIC, AICAR, AICARFT, IMPCHASE, PURH

Gene ID: 471

UniProt: P31939