DCBLD2 antibody (AA 399-416)

Catalog No. ABIN214270

This Rabbit Polyclonal antibody specifically detects DCBLD2 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Hamster.

Quick Overview for DCBLD2 antibody (AA 399-416) (ABIN214270)

Target: DCBLD2 (Discoidin, CUB and LCCL Domain Containing 2 (DCBLD2))

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCBLD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DCBLD2 Antibody

Binding Specificity: AA 399-416

Brand: IHC-plus™

Specificity: Residues 399-416 [QDKIFQGNKDYHKDVRNN] of the human ESDN protein

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%) Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (94%) Monkey, Platypus (89%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human DCBLD2. Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%), Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (94%), Monkey, Platypus (89%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for DCBLD2

Target: DCBLD2 (Discoidin, CUB and LCCL Domain Containing 2 (DCBLD2))

Alternative Name: DCBLD2

Background: Name/Gene ID: DCBLD2

Synonyms: DCBLD2, CLCP1, ESDN

Gene ID: 131566

UniProt: Q96PD2