S100 Protein (S100) antibody

Catalog No. ABIN214427

The Rabbit Polyclonal anti-S100 Protein antibody is suitable to detect S100 Protein in samples from Human and Cow. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for S100 Protein (S100) antibody (ABIN214427)

Target: S100 Protein (S100)

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Specificity: S100 protein

Purification: Protein A purified

Immunogen: Full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details

Target: S100 Protein (S100)

Alternative Name: S100 Protein

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins