CD9 antibody (FITC)

Catalog No. ABIN2144480

The Mouse Monoclonal anti-CD9 antibody has been validated for FACS and IF. It is suitable to detect CD9 in samples from Human.

Quick Overview for CD9 antibody (FITC) (ABIN2144480)

Target: CD9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 4AHI9a

Product Details anti-CD9 Antibody (FITC)

Characteristics: 4AHI9a reacts with CD9 ( p24), a member of the tetraspan ( TM4SF ) family with 24 kDa MW, expressed on platelets and weakly on B-cells. It also expressed on eosinophils, basophils, endothelial and epithelial cells. CD9 antigen modulates cell adhesion, migration and platelet activation. GM1CD9 triggers platelet activation resulted in platelet aggregation, but it is blocked by anti-Fc receptor antibody CD32. This clone is cross reactive with non-human primate.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Procedure:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD9

Target: CD9

Alternative Name: CD9

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization