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CD9 antibody (FITC)

The Mouse Monoclonal anti-CD9 antibody has been validated for FACS and IF. It is suitable to detect CD9 in samples from Human.
Catalog No. ABIN2144480

Quick Overview for CD9 antibody (FITC) (ABIN2144480)

Target

See all CD9 Antibodies
CD9

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD9 antibody is conjugated to FITC

Application

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Flow Cytometry (FACS), Immunofluorescence (IF)

Clone

4AHI9a
  • Characteristics

    4AHI9a reacts with CD9 ( p24), a member of the tetraspan ( TM4SF ) family with 24 kDa MW, expressed on platelets and weakly on B-cells. It also expressed on eosinophils, basophils, endothelial and epithelial cells. CD9 antigen modulates cell adhesion, migration and platelet activation. GM1CD9 triggers platelet activation resulted in platelet aggregation, but it is blocked by anti-Fc receptor antibody CD32. This clone is cross reactive with non-human primate.

    Isotype

    IgG1
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Assay Procedure

    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C
  • Target

    CD9

    Alternative Name

    CD9

    Pathways

    Response to Water Deprivation, Cell-Cell Junction Organization
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