CD147 antibody (PE)

Catalog No. ABIN2145003

This anti-CD147 antibody is a Mouse Monoclonal antibody detecting CD147 in WB, FACS, IHC and IF. Suitable for Human.

Quick Overview for CD147 antibody (PE) (ABIN2145003)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is conjugated to PE

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 4AHIM6

Product Details anti-CD147 Antibody (PE)

Characteristics: 4AHIM6 monoclonal antibody reacts with basigin or neurothelin, a 50-60 kDa transmembrane glycoprotein, broadly expressed on cells of hematopoietic and non-hematopoietic origin. Neutrothelin is a blood-brain barrier-specific molecule. CD147 play a role in embryonal blood barrier development and a role in integrin-mediated adhesion in brain endothelia.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Procedure:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147

Pathways: S100 Proteins