MADD antibody (C-Term)

Catalog No. ABIN214509

This anti-MADD antibody is a Rabbit Polyclonal antibody detecting MADD in WB, IHC, ICC and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for MADD antibody (C-Term) (ABIN214509)

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MADD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MADD Antibody

Binding Specificity: C-Term

Purification: Ion exchange chromatography

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (2.5 μg/mL), WB (1:250 - 1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MADD

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Alternative Name: DENN / MADD

Background: Name/Gene ID: MADD

Synonyms: MADD, DENN, IG20, KIAA0358, Rab3 GDP/GTP exchange factor, RAB3GEP

Gene ID: 8567

UniProt: Q8WXG6

Pathways: Caspase Cascade in Apoptosis