CDK1 antibody

Catalog No. ABIN215066

The Mouse Monoclonal anti-CDK1 antibody has been validated for WB, IHC, IF, IP, IHC (p), IHC (fro) and Func. It is suitable to detect CDK1 in samples from Human, Mouse, Rat, Chicken and Xenopus laevis.

Quick Overview for CDK1 antibody (ABIN215066)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Mouse, Rat, Chicken, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Product Details anti-CDK1 Antibody

Specificity: Recognizes a 34kD protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks). Human, mouse, rat, chicken, Xenopus.

Purification: Purified

Immunogen: Xenopus p34cdc2.

Isotype: IgG2a

Application Details

Application Notes: Approved: Func, IF, IHC, IHC-Fr, IHC-P, IP, WB (2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Immunoprecipitation (Native, denatured): 2 μg/mg protein lysate using Protein A. Immunohistochemistry (formalin/paraffin, frozen): 2-4 μg/mL for 30 minutes at RT. Paraffin sections require antigen retrieval. Western Blot: 2 μg/mL for 2 hours at RT. Positive control: HeLa or NIH3T3 cells, germinal center in a tonsil or reactive lymph node.

Comment:

Target Species of Antibody: Xenopus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1 / CDC2

Background: Name/Gene ID: CDK1
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

Gene ID: 983

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA