Hsc70 antibody

Catalog No. ABIN215208

This anti-Hsc70 antibody is a Rat Monoclonal antibody detecting Hsc70 in WB, IHC, IP, ICC and FACS. Suitable for Human, Rat, Mouse, Cow, Chicken, Dog, Sheep, Pig, Rabbit, Guinea Pig and Monkey.

Quick Overview for Hsc70 antibody (ABIN215208)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human, Rat, Mouse, Cow, Chicken, Dog, Sheep, Pig, Rabbit, Guinea Pig, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Hsc70 Antibody

Specificity: Specific for Hsc70 under both native and denaturing conditions. Detects a ~73kD protein, corresponding to the apparent molecular mass of Hsc70 on SDS-PAGE immunoblots. Species cross-reactivity: human, monkey, mouse, hamster, guinea pig, rat, rabbit, bovine, sheep, porcine, canine and chicken. Will not react with Hsc70 ATPase fragment or Hsp70.

Purification: Protein G purified

Immunogen: Hsc70 purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, ICC (5 μg/mL), IHC (1:200), IP (5 μg/mL), WB

Usage: Suitable for use in Western Blot (ECL), Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, FACS and Immunoelectron Microscopy. Western Blot (Colorimetric): 0.1 μg/mL. Immunoprecipitation: 5 μg/mL. Immunohistochemistry: 1:200. Immunocytochemistry: 5 μg/mL. Immunoelectron Microscopy: 1:1000. Positive control: Recombinant Bovine Hsc70 Protein.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF in 50 % glycerol.

Preservative: PMSF

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: HSPA8 / HSC70

Background: Name/Gene ID: HSPA8

Synonyms: HSPA8, Heat shock 70 kDa protein 8, Heat shock 70kd protein 10, Heat shock 70kD protein 8, Heat shock cognate protein 54, HSP71, HSPA10, HSC71, HSP73, LPS-associated protein 1, Heat shock 70  kDa protein 8, NIP71, HSC54, HSC70, LAP1

Gene ID: 3312

UniProt: P11142