TCP1 alpha/CCTA antibody (C-Term)

Catalog No. ABIN215497

This Rat Monoclonal antibody specifically detects TCP1 alpha/CCTA in WB, IP and ICC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit, Hamster, Monkey, Guinea Pig, Pig and Drosophila melanogaster.

Quick Overview for TCP1 alpha/CCTA antibody (C-Term) (ABIN215497)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Hamster, Monkey, Guinea Pig, Pig, Drosophila melanogaster

Host: Rat

Clonality: Monoclonal

Conjugate: This TCP1 alpha/CCTA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-TCP1 alpha/CCTA Antibody

Binding Specificity: C-Term

Specificity: This affinity purified antibody recognizes an epitope in the C-terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60kD protein, corresponding to the apparent molecular mass of TCP-1alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, porcine and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit. In plants, CTA-191 recognizes TCP-1 of Pisum sativum and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.

Purification: Protein G purified

Immunogen: A purified recombinant mouse TCP-1alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli.

Isotype: IgG2a

Application Details

Application Notes: Approved: ICC (5 μg/mL), IP (12.5 μg/mL), WB

Usage: Western Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 12.5 μg/mL 5 μg/mL. Immunocytochemistry: 5 μg/mL. Positive control: HeLa Heat Shocked Cell Lysate.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, in 50 % glycerol.

Preservative: PMSF

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Alternative Name: TCP1

Background: Name/Gene ID: TCP1

Synonyms: TCP1, CCTA, CCT-alpha, D6S230E, T-complex 1, TCP-1-alpha, Tailless complex polypeptide 1, CCT1

Gene ID: 6950

UniProt: P17987