ADORA1 antibody (AA 163-176)

Catalog No. ABIN215585

The Rabbit Polyclonal anti-ADORA1 antibody has been validated for WB, ELISA, IHC and IHC (fro). It is suitable to detect ADORA1 in samples from Rat.

Quick Overview for ADORA1 antibody (AA 163-176) (ABIN215585)

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADORA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ADORA1 Antibody

Binding Specificity: AA 163-176

Specificity: Recognizes Adenosine A1 receptor (A1R) at79kD and 39kD bands of in cortical membranes. The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent a A1R dimers. Species cross-reactivity: Human, rat, porcine and sheep. Species Homology: Canine and rabbit- 92 % , bovine-78 %.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Dog, Rabbit, Guinea pig (93%) Gibbon, Panda, Horse, Pig (86%).

Purification: Immunoaffinity purified

Immunogen: Sequence corresponding to the 3rd extracellular domain (aa163-176, GEPVIKAEFEKVIS) of the rat A1R gene. Percent identity by BLAST analysis: Human, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Dog, Rabbit, Guinea pig (93%), Gibbon, Panda, Horse, Pig (86%).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IHC, IHC-Fr, WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:10000-1:50000. Western Blot (ECL): 1-10 μg/mL. Higher concentrations of antibody may be necessary if colorimetric detection or milk-based antibody diluent are used. Immunohistochemistry (Frozen): 10 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % BSA

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Alternative Name: ADORA1 / Adenosine A1 Receptor

Background: Name/Gene ID: ADORA1
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA1, A1 adenosine receptor, A1ar, Adenosine A1 receptor, Adenosine receptor A1, ADO-A1 receptor, RDC7

Gene ID: 134

UniProt: P30542

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane