ADORA1 antibody (3rd Intracellular Loop)

Catalog No. ABIN7042882

This Rabbit Polyclonal antibody specifically detects ADORA1 in WB, IHC, IF and IC. It exhibits reactivity toward Human.

Quick Overview for ADORA1 antibody (3rd Intracellular Loop) (ABIN7042882)

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADORA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-ADORA1 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 213-229

Purpose: A Rabbit Polyclonal Antibody to Adenosine A1 Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat,bovine,canis,pig - identical

Characteristics: Anti-Adenosine A1 Receptor Antibody is directed against human adenosine A1 receptor. Anti-Adenosine A1 Receptor Antibody (ABIN7042882, ABIN7043894 and ABIN7043895) can be used in western blot analysis, immunocytochemical and immunohistochemical applications. It has been designed to recognize adenosine A1R from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234623)

Blocking Peptide: (ABIN7234623)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Alternative Name: ADORA1

Background:

Synonyms: ADORA1, A1AR, Adenosine A1R

Description: Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3A1AR is widely distributed and has been well characterized. High expression of A1AR is found in the brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, the colon, and kidney.2,4A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG).5Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem.6,7 A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.8-10

Gene ID: 134

NCBI Accession: NM_000674

UniProt: P30542

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane