Adenosine A2a Receptor antibody (C-Term)

Catalog No. ABIN215588

The Rabbit Polyclonal anti-Adenosine A2a Receptor antibody has been validated for WB, ELISA, IHC, IF and IHC (fro). It is suitable to detect Adenosine A2a Receptor in samples from Human, Rat, Dog, Cow and Pig.

Quick Overview for Adenosine A2a Receptor antibody (C-Term) (ABIN215588)

Target: Adenosine A2a Receptor (ADORA2A)

Reactivity: Human, Rat, Dog, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A2a Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Adenosine A2a Receptor Antibody

Binding Specificity: C-Term

Specificity: Recognizes canine Adenosine A2a receptor. Recognizes a band of ~45kD in human (coronary artery), and rat, porcine and bovine striatal membranes. Species cross-reactivity: human, rat, bovine and porcine.

Purification: Immunoaffinity purified

Immunogen: Amino acid sequence near the 4th C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected is not present in A1, A2b and A3 receptors.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IF, IHC, IHC-Fr (10 - 50 μg/mL), WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 1:10000-1:50000. Immunohistochemistry (Frozen): 10-50 μg/mL. Western Blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.

Comment:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: ADORA2A/Adenosine A2A Receptor

Background: Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8

Gene ID: 135

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints