GJB1 antibody (Cytoplasmic Domain)

Catalog No. ABIN215715

This anti-GJB1 antibody is a Rabbit Polyclonal antibody detecting GJB1 in WB, IHC, ELISA and IHC (fro). Suitable for Human, Rat and Mouse.

Quick Overview for GJB1 antibody (Cytoplasmic Domain) (ABIN215715)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GJB1 Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Reacts predominantly with rat Connexin 32. Cross-reactive bands may be detected in the 50-65kD range. These bands may represent Connexin 32 dimers, but this has not been confirmed. Species cross-reactivity: human, mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to a portion of the cytoplasmic loop of rat connexin 32.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr, WB (0.5 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1.0 μg/mL. Western Blot: 0.5 μg/mL. Detects a 32kD protein in rat liver preparations enriched for integral membrane proteins. Note: samples were not boiled prior to running SDS-PAGE. Immunohistochemistry (Frozen sections: 8,11): 1-2 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1 / CX32 / Connexin 32

Background: Name/Gene ID: GJB1
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJB1, Connexin 32, CMTX1, Connexin-32, CX32, Gap junction beta-1 protein, Gap junction protein beta-1, CMTX

Gene ID: 2705

UniProt: P08034

Pathways: Cell-Cell Junction Organization