Cytokeratin 13 antibody

Catalog No. ABIN215740

This Mouse Monoclonal antibody specifically detects Cytokeratin 13 in WB, IHC, IHC (p), IF and IHC (fro). It exhibits reactivity toward Human, Rat and Cow.

Quick Overview for Cytokeratin 13 antibody (ABIN215740)

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cytokeratin 13 Antibody

Specificity: Antigen Recognized in Human, bovine and rat. Reactivities on Cultured Cell lines from squamous cell CA. Cell Lines (tested so far) e. g. A-431 from epidermoid CA of vulva, RT 112, RT-4 of urinary bladder. Represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54 000 polypeptide (human cytokeratin 13 with minor affinity to cytokeratins 14, Mr 50000, and 16, Mr 48000. Tumors specifically detected: several squamous cell carcinomas, e. g. cervix carcinoma, transitional cell carcinoma of the bladder, craniopharyngioma.

Purification: Protein A purified

Immunogen: Cytokeratin 13 of Mr 54000 purified from human esophagus.

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Immunofluorescence, Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material, protease pretreatment improves the performance of this excellent marker) and Western Blot. Immunohistochemistry: 1:10 with PBS,  pH 7.4, Incubation Time 1 hr at RT, extended with paraffin.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Alternative Name: KRT13 / CK13 / Cytokeratin 13

Background: Name/Gene ID: KRT13

Synonyms: KRT13, CK13, Cytokeratin 13, Cytokeratin-13, CK-13, K13, Keratin 13, Keratin-13

Gene ID: 3860