Cytokeratin 19 antibody (AA 352-368)

Catalog No. ABIN215749

This Mouse Monoclonal antibody specifically detects Cytokeratin 19 in WB, IHC, ELISA and IHC (fro). It exhibits reactivity toward Human and Monkey.

Quick Overview for Cytokeratin 19 antibody (AA 352-368) (ABIN215749)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cytokeratin 19 Antibody

Binding Specificity: AA 352-368

Specificity: Antigen Recognized in Human, rat, fish, bovine, Species (tested so far) not with mouse, woodchuck, chicken, xenopus. Reactivities on Cultured Cell Lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12. Polypeptide Reacting: Mr 40 000 polypeptide (cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa352-368 (VRADSERQNQEYQRLMD) of the a-helical fragment.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (88%) Mouse, Horse (82%).

Purification: Protein A purified

Immunogen: Cytokeratin 19 of Mr 40000, from cultured human MCF-7 cells. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (88%), Mouse, Horse (82%).

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, WB

Usage: Suitable for Immunohistochemistry (frozen tissue), Western Blot (Western) and ELISA. Immunohistochemistry: 1:10 with PBS,  pH 7.4, Incubation Time 1 hr at RT.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: KRT19 / CK19 / Cytokeratin 19

Background: Name/Gene ID: KRT19

Synonyms: KRT19, CK19, Cytokeratin 19, Cytokeratin-19, Keratin 19, Keratin-19, CK-19, K19, K1CS, Keratin, type I, 40-kd

Gene ID: 3880

UniProt: P08727