GDF5 antibody

Catalog No. ABIN217376

The Rat Monoclonal anti-GDF5 antibody has been validated for ELISA, Neut and Func. It is suitable to detect GDF5 in samples from Mouse.

Quick Overview for GDF5 antibody (ABIN217376)

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GDF5 antibody is un-conjugated

Application: ELISA, Neutralization (Neut), Functional Studies (Func)

Product Details anti-GDF5 Antibody

Specificity: Recognizes mouse GDF-5 in direct ELISA. Does not cross-react with rmGDF-1, rmGDF-3, or rmGDF-8.

Purification: Protein G purified

Immunogen: E. coli-derived monomeric rmGDF-5.

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Func, Neut

Usage: Suitable for use in direct ELISA and Neutralization. Neutralization (ND50): ~2-10 μg/mL in the presence of 3 μg/mL of rmGDF-5, using the ATDC5 cell line. Note: The exact concentration of antibody required to neutralize rmGDF-5 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~10 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Storage: -20 °C

Storage Comment: Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.

Target Details for GDF5

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Alternative Name: GDF5 / GDF-5

Background: Name/Gene ID: GDF5
Family: TGF beta

Synonyms: GDF5, CDMP-1, CDMP1, GDF-5, LAP4, Radotermin, OS5, BMP14, SYNS2

Gene ID: 8200

UniProt: P43026