Rubella Virus Glycoprotein E1 (AA 801-900) antibody (Biotin)

Catalog No. ABIN2173788

The Biotin-conjugated Rabbit Polyclonal anti-Rubella Virus Glycoprotein E1 antibody (ABIN2173788) specifically detects Rubella Virus Glycoprotein E1 in ELISA, IHC (fro) and IHC (p). The antibody is reactive with Rubella Virus (RuV) and Virus samples.

Quick Overview for Rubella Virus Glycoprotein E1 (AA 801-900) antibody (Biotin) (ABIN2173788)

Target: Rubella Virus Glycoprotein E1

Reactivity: Rubella Virus (RuV), Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 801-900

Cross-Reactivity: Virus

Cross-Reactivity (Details): Rubella virus

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Rubella Virus E1 envelope glycoprotein

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details

Target: Rubella Virus Glycoprotein E1

Alternative Name: E1 glycoprotein

Target Type: Viral Protein

Background:

Synonyms: E1, E1 envelope glycoprotein, Glycoprotein E1, Spike glycoprotein E1, POLS_RUBVM.

Background: Rubella virus (RV), the sole member of the genus Rubivirus within the family Togaviridae, is a small enveloped, positive strand RNA virus. The nucleocapsid consists of 40S genomic RNA and a single species of capsid protein which is enveloped within a host-derived lipid bilayer containing two viral glycoproteins, E1 (58 kDa) and E2 (42-46 kDa). In virus infected cells, RV matures by budding either at the plasma membrane, or at the internal membranes depending on the cell type and enters adjacent uninfected cells by a membrane fusion process in the endosome, directed by E1-E2 heterodimers. The heterodimer formation is crucial for E1 transport out of the endoplasmic reticulum to the Golgi and plasma membrane. In RV E1, a cysteine at position 82 is crucial for the E1-E2 heterodimer formation and cell surface expression of the two proteins. E1 has been shown to be a type 1 membrane protein, rich in cysteine residues with extensive intramolecular disulphide bonds [PMID: 11682134]