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GPR84 antibody (AA 21-120)

Name: GPR84 antibody (AA 21-120)
SKU: ABIN2173922
Price: 384.62 USD
Availability: InStock

The Rabbit Polyclonal anti-GPR84 antibody has been validated for WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p) and IHC (fro). It is suitable to detect GPR84 in samples from Human, Mouse and Rabbit.

Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 antibody (AA 21-120))

Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 antibody (AA 21-120))

Paraformaldehyde-fixed, paraffin embedded human glioma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (GPR84 antibody (AA 21-120))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (GPR84 antibody (AA 21-120))

IHC (p)

Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (GPR84 antibody (AA 21-120))

FACS

5 images

Catalog No. ABIN2173922

Quick Overview for GPR84 antibody (AA 21-120) (ABIN2173922)

Target

See all GPR84 Antibodies

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Reactivity

Human, Mouse, Rabbit

Host

Rabbit

Clonality

Polyclonal

Conjugate

This GPR84 antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Binding Specificity
16

7

5

3

3

2

2

1

1

1

1

1

1

1

1

1
AA 21-120

Cross-Reactivity

Human, Mouse, Rabbit

Predicted Reactivity

Cow,Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human GPCR EX33 protein

Isotype

IgG
anti-G Protein-Coupled Receptor 84 (GPR84) (Cytoplasmic Domain) antibody
GPR84 Reactivity: Human ELISA, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 5C3 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 166-180), (Extracellular) antibody
GPR84 Reactivity: Human WB, FACS, IHC, LCI, IF Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (N-Term) antibody
GPR84 Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Transmembrane Domain) antibody
GPR84 Reactivity: Human IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Extracellular Domain) antibody
GPR84 Reactivity: Human, Horse, Monkey IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 202-320) antibody
GPR84 Reactivity: Human ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 1-100) antibody
GPR84 Reactivity: Mouse WB, ELISA, FACS Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 265-293) antibody
GPR84 Reactivity: Human WB Host: Rabbit Polyclonal RB36053 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Alternative Name

GPCR EX33

Background

Synonyms: EX33, GPCR4, G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33, GPR84
Background: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

Gene ID

53831

UniProt

Q9NQS5