GPR84 antibody (2nd Extracellular Loop) (PE)

Catalog No. ABIN7237670

The PE-conjugated Rabbit Polyclonal anti-GPR84 antibody (ABIN7237670) specifically detects GPR84 in FACS and LCI. The antibody is reactive with Human samples.

Quick Overview for GPR84 antibody (2nd Extracellular Loop) (PE) (ABIN7237670)

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR84 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR84 Antibody (PE)

Binding Specificity: 2nd Extracellular Loop, AA 166-180

Purpose: A Rabbit Polyclonal Antibody to GPR84 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - 14,15 amino acid residues identical, mouse,rat - 13

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen: CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582046)

Blocking Peptide: (ABIN7235454)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR84

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Alternative Name: GPR84

Background:

Synonyms: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Description: GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.

Gene ID: 53831

NCBI Accession: NM_020370

UniProt: Q9NQS5