FOXA2 antibody (AA 201-300) (PE)

Catalog No. ABIN2174177

The Rabbit Polyclonal anti-FOXA2 antibody has been validated for WB and FACS. It is suitable to detect FOXA2 in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for FOXA2 antibody (AA 201-300) (PE) (ABIN2174177)

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXA2 antibody is conjugated to PE

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-FOXA2 Antibody (PE)

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HNF 3 beta

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-FOXA2 antibody (PE) (ABIN2174177)

Yamamizu, Schlessinger, Ko: "SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1)." in: Development (Cambridge, England), Vol. 141, Issue 22, pp. 4254-66, (2014) (PubMed).

Yamamizu, Fujihara, Tachibana, Katayama, Takahashi, Hara, Imai, Shinkai, Yamashita: "Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a." in: Cell stem cell, Vol. 10, Issue 6, pp. 759-70, (2012) (PubMed).

Target Details for FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Alternative Name: Foxa2/Hnf 3beta

Background:

Synonyms: HNF3B, TCF3B, Hepatocyte nuclear factor 3-beta, HNF-3-beta, HNF-3B, Forkhead box protein A2, Transcription factor 3B, TCF-3B, FOXA2

Background: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs, FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis, regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Gene ID: 3170

UniProt: Q9Y261

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process