HHV DNA Polymerase Catalytic Subunit (AA 1131-1235) antibody

Catalog No. ABIN2175764

The Rabbit Polyclonal anti-HHV DNA Polymerase Catalytic Subunit antibody is suitable to detect HHV DNA Polymerase Catalytic Subunit in samples from Human Herpesvirus 1 (HHV-1) and Virus. It has been validated for WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) and ICC.

Quick Overview for HHV DNA Polymerase Catalytic Subunit (AA 1131-1235) antibody (ABIN2175764)

Target: HHV DNA Polymerase Catalytic Subunit

Reactivity: Human Herpesvirus 1 (HHV-1), Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 1131-1235

Specificity: This antibody will detect both HHV1 and HHV2 DNA polymerase catalytic subunit

Cross-Reactivity: Virus

Cross-Reactivity (Details): HHV1

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Human herpesvirus 1 DNA polymerase catalytic subunit

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: HHV DNA Polymerase Catalytic Subunit

Target Type: Viral Protein

Background:

Synonyms: DPOL_HHV11, DNA polymerase catalytic subunit, UL30, HHV1gp046.

Background: Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication.