CD31 antibody

Catalog No. ABIN217616

The Mouse Monoclonal anti-CD31 antibody is suitable to detect CD31 in samples from Rat. It has been validated for FACS, WB, IHC, ELISA and IHC (fro).

Quick Overview for CD31 antibody (ABIN217616)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: TLD-3A12

Product Details anti-CD31 Antibody

Specificity: Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2 M boric acid,  pH 7.0. Has been shown to be cross-reactive with endothelial cells derived from rhesus macaque.

Purification: Protein G purified

Endotoxin Level: Low Endotoxin

Immunogen: Activated, Lewis rat derived microglial cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:50 - 1:100), IHC, IHC-Fr, WB

Usage: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM-1 / CD31

Background: Name/Gene ID: PECAM1

Synonyms: PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA

Gene ID: 5175

Pathways: Regulation of Actin Filament Polymerization