IL-10 antibody

Catalog No. ABIN217691

The Rat Monoclonal anti-IL-10 antibody has been validated for WB, IHC, ELISA, FACS and IHC (p). It is suitable to detect IL-10 in samples from Human.

Quick Overview for IL-10 antibody (ABIN217691)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: JES3-12G8

Product Details anti-IL-10 Antibody

Specificity: Specific for human Interleukin-10 (IL-10). IL-10 is expressed by activated CD8+ peripheral blood T-cells, activated T-helper CD4+ T cell clones mast cells, B cell lymphomas and LPS activated monocytes. This clone is reported to neutralize the effects of IL-10(4).

Purification: Protein G purified

Immunogen: Human IL-10 expressed by COS cells.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-P (1:100), WB (1:100 - 1:250)

Usage: Flow Cytometry: Membrane permeabilization is required for this application. Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Immunohistology: This product requires antigen retrieval using heat treatment with sodium citrate buffer  pH 6.0 prior to staining paraffin sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Background: Name/Gene ID: IL10
Family: Hormone

Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF

Gene ID: 3586

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints