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CD28 antibody

This Mouse Monoclonal antibody specifically detects CD28 in FACS and Func. It exhibits reactivity toward Mouse.
Catalog No. ABIN217765

Quick Overview for CD28 antibody (ABIN217765)

Target

See all CD28 Antibodies
CD28

Reactivity

  • 194
  • 113
  • 52
  • 38
  • 22
  • 17
  • 13
  • 12
  • 7
  • 7
  • 7
  • 6
  • 1
  • 1
  • 1
  • 1
Mouse

Host

  • 154
  • 81
  • 28
  • 14
  • 12
  • 3
  • 2
  • 1
  • 1
Mouse

Clonality

  • 218
  • 71
  • 4
Monoclonal

Conjugate

  • 110
  • 32
  • 29
  • 20
  • 14
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CD28 antibody is un-conjugated

Application

  • 231
  • 92
  • 62
  • 50
  • 39
  • 39
  • 38
  • 26
  • 22
  • 22
  • 13
  • 7
  • 6
  • 6
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Functional Studies (Func)
  • Specificity

    Recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.

    Purification

    Protein G purified

    Endotoxin Level

    Low Endotoxin

    Immunogen

    Alternate immunization with A20 cells expressing mCD28 and recombinant mCD28Ig.

    Isotype

    IgG2b
  • Application Notes

    Approved: Flo (1:50 - 1:100), Func

    Usage: Flow Cytometry: Use10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4.

    Handling Advice

    Aliquot to Avoid freeze/thaw cycles.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot to avoid freeze/thaw cycles.
  • Target

    CD28

    Alternative Name

    CD28

    Background

    Name/Gene ID: CD28

    Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

    Gene ID

    940

    Pathways

    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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