Nephrin antibody (AA 451-550)

Catalog No. ABIN2177857

This Rabbit Polyclonal antibody specifically detects Nephrin in FACS. It exhibits reactivity toward Human, Mouse, Rat and Dog and has been mentioned in 2+ publications.

Quick Overview for Nephrin antibody (AA 451-550) (ABIN2177857)

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nephrin antibody is un-conjugated

Application: Flow Cytometry (FACS)

Product Details anti-Nephrin Antibody

Binding Specificity: AA 451-550

Cross-Reactivity: Human

Predicted Reactivity: Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nephrin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Nephrin antibody (ABIN2177857)

Wang, Feng, He, Sun, He, Jin: "miR-188-3p abolishes germacrone-mediated podocyte protection in a mouse model of diabetic nephropathy in type I diabetes through triggering mitochondrial injury." in: Bioengineered, (2021) (PubMed).

Chen, Cheng, Chen, Shao, Sung, Chiang, Yang, Lin, Sun, Sheu, Chang, Lee, Yip: "Combination therapy of exendin-4 and allogenic adipose-derived mesenchymal stem cell preserved renal function in a chronic kidney disease and sepsis syndrome setting in rats." in: Oncotarget, Vol. 8, Issue 59, pp. 100002-100020, (2018) (PubMed).

Target Details for Nephrin

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Alternative Name: Nephrin

Background:

Synonyms: CNF, NPHN, nephrin, Renal glomerulus-specific cell adhesion receptor, NPHS1

Background: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

Gene ID: 4868

UniProt: O60500

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development