CD28 antibody

Catalog No. ABIN217792

This Rat Monoclonal antibody specifically detects CD28 in FACS, IHC, Func and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD28 antibody (ABIN217792)

Target: CD28

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD28 Antibody

Specificity: Recognizes the human CD28 cell surface antigen, a 44kD glycoprotein expressed by T lymphocytes. The antibody enhances the MLR response. It has been reported that this Recognizes an epitope of CD28 expressed by NK cells, which is not recognized by clones of 9.3 or CD28.2(2).

Purification: Protein G purified

Endotoxin Level: Low Endotoxin

Immunogen: Human peripheral blood T-cells.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo (1:50 - 1:100), Func, IHC, IHC-Fr

Usage: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: Name/Gene ID: CD28

Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

Gene ID: 940

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response