NEFH antibody (AA 21-120)

Catalog No. ABIN2177936

The Rabbit Polyclonal anti-NEFH antibody has been validated for WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p) and IF (cc). It is suitable to detect NEFH in samples from Human, Rat and Chicken. There are 2+ publications available.

Quick Overview for NEFH antibody (AA 21-120) (ABIN2177936)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-NEFH Antibody

Binding Specificity: AA 21-120

Cross-Reactivity: Chicken, Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NF-H

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NEFH antibody (ABIN2177936)

Liu, Zhang, Zhang, Zhang, Hao, Song, Li, Feng, Hao, Shen, Min, Yang, Zhao: "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." in: Toxicology letters, Vol. 256, pp. 19-32, (2016) (PubMed).

Liu, Nie, Zhao, Zhang, Zhang, Wang, Cheng: "Conserved dopamine neurotrophic factor-transduced mesenchymal stem cells promote axon regeneration and functional recovery of injured sciatic nerve." in: PLoS ONE, Vol. 9, Issue 10, pp. e110993, (2014) (PubMed).

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: NF-H

Background:

Synonyms: NFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, KIAA0845

Background: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

Gene ID: 4744

UniProt: P12036