Podocin antibody (AA 231-330) (FITC)

Catalog No. ABIN2178530

The Rabbit Polyclonal anti-Podocin antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect Podocin in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for Podocin antibody (AA 231-330) (FITC) (ABIN2178530)

Target: Podocin (NPHS2) (Nephrosis 2, Idiopathic, Steroid-Resistant (Podocin) (NPHS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Podocin antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Podocin Antibody (FITC)

Binding Specificity: AA 231-330

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Podocin

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Podocin antibody (FITC) (ABIN2178530)

Ishida, Kami, Kusaba, Kirita, Kishida, Mazda, Adachi, Gojo: "Kidney-specific Sonoporation-mediated Gene Transfer." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 24, Issue 1, pp. 125-34, (2016) (PubMed).

Jayachandran, Lugo, Heiling, Miller, Rule, Lieske: "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." in: Biology of sex differences, Vol. 6, pp. 2, (2015) (PubMed).

Target Details for Podocin

Target: Podocin (NPHS2) (Nephrosis 2, Idiopathic, Steroid-Resistant (Podocin) (NPHS2))

Alternative Name: Podocin

Background:

Synonyms: PDCN, SRN1, Podocin, NPHS2

Background: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

Gene ID: 7827

UniProt: Q9NP85