PRMT6 antibody (AA 23-43)

Catalog No. ABIN218313

The Rabbit Polyclonal anti-PRMT6 antibody (ABIN218313) specifically detects PRMT6 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse and Monkey samples.

Quick Overview for PRMT6 antibody (AA 23-43) (ABIN218313)

Target: PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PRMT6 Antibody

Binding Specificity: AA 23-43

Specificity: Synthetic peptide of human PRMT6.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Marmoset (100%) Orangutan (95%) Elephant (86%) Horse (81%).

Purification: Protein G purified

Immunogen: This antibody was developed a synthetic peptide corresponding to amino acids 23-43 (C-EEDGAEREAALERPRRTKRER) of human PRMT6, GenBank no. gb|AAH73866.1|. HeLa cell lysate (IMGENEX, cat. no. 40161) can be used as positive control. This antibody also cross reacts with mouse cell line (RAW). Percent identity by BLAST analysis: Human, Monkey, Marmoset (100%), Orangutan (95%), Elephant (86%), Horse (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Short term: 4°C
Long term: -70°C.

Target Details for PRMT6

Target: PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))

Alternative Name: PRMT6

Background: Name/Gene ID: PRMT6

Synonyms: PRMT6, Arginine methyltransferase 6, HRMT1L6

Gene ID: 55170

UniProt: Q96LA8