LY86 antibody

Catalog No. ABIN2191781

The Rat Monoclonal anti-LY86 antibody has been validated for FACS and Func. It is suitable to detect LY86 in samples from Mouse. There is 1 publication available.

Quick Overview for LY86 antibody (ABIN2191781)

Target: LY86 (Lymphocyte Antigen 86 (LY86))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LY86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: MD113

Product Details anti-LY86 Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-LY86 antibody (ABIN2191781)

Nagai, Shimazu, Ogata, Akashi, Sudo, Yamasaki, Hayashi, Iwakura, Kimoto, Miyake: "Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide." in: Blood, Vol. 99, Issue 5, pp. 1699-705, (2002) (PubMed).

Target Details for LY86

Target: LY86 (Lymphocyte Antigen 86 (LY86))

Alternative Name: Md-1

Background: The monoclonal antibody MD113 reacts with mouse MD-1. MD-1 (22-25 kD) is an extracellular protein associated with the extracellular domain of RP105. The latter is a type 1 transmembrane protein with leucine-rich repeats. RP105 is similar to Drosophila Toll. MD-1 associated with RP105 (similar to TLR4/MD-2) appears to be an essential molecule that helps RP105 on B-cells to signal to LPS. The MD113 antibody is antagonistic in LPS-induced B-cell proliferation and B7.2 up-regulation.

Pathways: Toll-Like Receptors Cascades