Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

MHC, Class I antibody

This anti-MHC, Class I antibody is a Rat Monoclonal antibody detecting MHC, Class I in FACS and IHC (fro). Suitable for Mouse.
Catalog No. ABIN2191839

Quick Overview for MHC, Class I antibody (ABIN2191839)

Target

See all MHC, Class I Antibodies
MHC, Class I

Reactivity

  • 72
  • 35
  • 13
  • 10
  • 7
  • 6
  • 5
  • 5
  • 5
  • 5
  • 4
  • 1
Mouse

Host

  • 56
  • 47
  • 6
  • 1
Rat

Clonality

  • 71
  • 36
  • 3
Monoclonal

Conjugate

  • 36
  • 14
  • 11
  • 8
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MHC, Class I antibody is un-conjugated

Application

  • 66
  • 53
  • 33
  • 24
  • 19
  • 15
  • 14
  • 13
  • 11
  • 11
  • 7
  • 5
  • 5
  • 3
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

ER-HR52
  • Sterility

    0.2 μm filtered
  • Application Notes

    For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

    Restrictions

    For Research Use only
  • Buffer

    PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

    Expiry Date

    12 months
  • Target

    MHC, Class I

    Alternative Name

    Mhc Class I

    Background

    The monoclonal antibody ER-HR52 specifically reacts with major histocompatibility complex (MHC) class I antigens of the mouse and, therefore, it is a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells. The primary immunological function of MHC molecules is to bind and present antigenic peptides on the surfaces of cells for recognition by the antigen-specific T cell receptors (TCRs) of lymphocytes. MHC class I molecules are expressed on the surfaces of most cells and are recognized by CD8-positive cytotoxic T-cells, an essential step for initiating the elimination of virally infected cells by T-cell-mediated lysis. MHC class I molecules are heterodimers composed of an alpha (44kD) and a beta (beta microglobulin, 11 kD) subunit. The first two structural domains of the alpha subunit associate to form the peptide-binding pocket. The monoclonal antibody ER-HR52 reacts with MHC class I, an antigen that is expressed by all somatic cells at varying levels. Lymphocytes are highly positive, whereas fibroblasts and neurons show only a low level of antigen expression. ER-HR52 recognizes murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Db, H-2Dw16, H-2d,p,q. Weaker reactivity is found in mice of the following haplotypes: H-2f,r,s,w17, w23,w27. MHC class I molecules of other haplotypes are not recognized by ER-MP52. There is no cross-reactivity with human MHC class I molecules.
You are here:
Chat with us!