Ficolin 2 antibody

Catalog No. ABIN2191965

Cited in 2+ publications. The Mouse Monoclonal anti-Ficolin 2 antibody (Clone GN4) (ABIN2191965) specifically detects Ficolin 2 in WB, IP, IHC (fro) and IA. The antibody is reactive with Human samples.

Quick Overview for Ficolin 2 antibody (ABIN2191965)

Target: Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ficolin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Clone: GN4

Product Details anti-Ficolin 2 Antibody

Cross-Reactivity (Details): Cross reactivity: Rat : Yes

Sterility: 0.2 μm filtered

Isotype: IgG1

Application Details

Application Notes: For immunohistochemistry, immunoprecipitation and immuno assays, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-Ficolin 2 antibody (ABIN2191965)

Matsushita, Kuraya, Hamasaki, Tsujimura, Shiraki, Fujita: "Activation of the lectin complement pathway by H-ficolin (Hakata antigen)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 7, pp. 3502-6, (2002) (PubMed).

Matsushita, Endo, Hamasaki, Fujita: "Activation of the lectin complement pathway by ficolins." in: International immunopharmacology, Vol. 1, Issue 3, pp. 359-63, (2001) (PubMed).

Target Details for Ficolin 2

Target: Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))

Alternative Name: Ficolin-2

Background: The monoclonal antibody GN4 recognizes human Ficolin-2, also known as L-ficolin. Ficolins are a family of pattern recognition molecules containing both a collagen-like domain and a fibrinogen-like domain that are capable of activating the complement system, through association with MASPs, on the surface of microorganisms. In human, three Ficolins are known: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin), and Ficolin-3 (H-ficolin). Ficolin-1, is mainly expressed by monocytes, granulocytes and lung cells and is present locally in areas of inflammation. Ficolin-2 (also L-ficolin, ficolin L, EBP-37 or hucolin) is produced primarily by the liver. The association between Ficolin-2 and MASPs/sMAP is Ca2+-dependent. MASPs in Ficolin-2 - MASP complexes have proteolytic activities for C4, C2 and C3. Ficolin-2 also shows opsonic activity. Aliases Ficolin-2/P35, L-ficolin, ficolin L, EBP-37 or hucolin

Pathways: Complement System