LGALS3
Reactivity: Human, Mouse
ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of galectin-3 to be inhibited.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin.
Storage
4 °C
Storage Comment
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date
12 months
Feilchenfeldt, Tötsch, Sheu, Robert, Spiliopoulos, Frilling, Schmid, Meier: "Expression of galectin-3 in normal and malignant thyroid tissue by quantitative PCR and immunohistochemistry." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 16, Issue 11, pp. 1117-23, (2003) (PubMed).
Sano, Hsu, Yu, Apgar, Kuwabara, Yamanaka, Hirashima, Liu: "Human galectin-3 is a novel chemoattractant for monocytes and macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 4, pp. 2156-64, (2000) (PubMed).
The monoclonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl- terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up- regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas. Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions. The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3's hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process. The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.