Chips antibody

Catalog No. ABIN2192149

This Mouse Monoclonal antibody specifically detects in IHC (p), WB, IHC (fro), Inhibition, IA and FACS. It exhibits reactivity toward Chemical.

Quick Overview for Chips antibody (ABIN2192149)

Target: Chips

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Antibody (Inhibition), Immunoassay (IA), Flow Cytometry (FACS)

Clone: JCC1

Product Details

Sterility: 0.2 μm filtered

Application Details

Application Notes: For immunohistology, Western blot and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10 for flow cytometry and 1:100 for Western blot and immunohistology. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.01 % sodium azide and 0.1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details

Target: Chips

Target Type: Chemical

Background: The bacterial pathogen Staphylococcus aureus is insensitive to antimicrobial host defense peptides such as defensins, protegrins, platelet microbicidal proteins and bacteriocins. Staphylococci have developed various resistance mechanisms including those specific for bacteriocins and several host defense peptides. A protein belonging to the resistance mechanism of Staphylococcus aureus is known as CHIPS (Chemotaxis Inhibiting Protein of Staphylococcus aureus). CHIPS is a protein produced by Staphylococcus aureus that inhibits chemotaxis of neutrophils by blocking the Formyl Peptide Receptor (FPR) and C5a Receptor on neutrophils. CHIPS and antibodies against CHIPS can be useful for various experimental infection models of Staphylococcus aureus. Furthermore these reagents can be of help in studies on the role of FPR and C5a in inflammatory processes. Monoclonal antibody JCC1 reacts with the C-terminus of CHIPS.