Cytotoxic Necrotizing Factor 1 antibody

Catalog No. ABIN2192151

This anti- antibody is a Mouse Monoclonal antibody detecting in DB, WB, IA and InhA. Suitable for Chemical. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Cytotoxic Necrotizing Factor 1 antibody (ABIN2192151)

Target: Cytotoxic Necrotizing Factor 1

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Western Blotting (WB), Immunoassay (IA), Inhibition Assay (InhA)

Clone: NG8

Product Details

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 to be inactivated.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

McNichol, Rasmussen, Carvalho, Meysick, OBrien: "Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein." in: Infection and immunity, Vol. 75, Issue 11, pp. 5095-104, (2007) (PubMed).

Meysick, Mills, OBrien: "Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli." in: Infection and immunity, Vol. 69, Issue 4, pp. 2066-74, (2001) (PubMed).

Target Details

Target: Cytotoxic Necrotizing Factor 1

Target Type: Chemical

Background: The monoclonal antibody NG8 is specific for Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic Escherichia coli. CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho, however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin. Monoclonal antibody NG8 recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.