IL-10 antibody

Catalog No. ABIN2192182

This Rabbit Polyclonal antibody specifically detects IL-10 in WB, Neut, IA and StM. It exhibits reactivity toward Human.

Quick Overview for IL-10 antibody (ABIN2192182)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut), Immunoassay (IA), Staining Methods (StM)

Product Details anti-IL-10 Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For cell staining dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IL-10 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: Il-10

Background: The antibody reacts with human Interleukin-10 (IL-10) of both natural and recombinant origin. IL-10 is a pluripotent cytokine with important immunosuppressive actions: it can inhibit cytokines involved in the Th1 response such as IL-2, Interferon gamma and also inhibits the production of pro-inflammatory cytokines such as IL-1, IL-6, IL-8 and TNF-alpha. The antibody inhibits the biological activity of natural and recombinant human IL-10. The antigen specificity was further assessed by ELISA. No cross reactivities with other cytokines have been detected.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints