Lactoferrin antibody

Catalog No. ABIN2192193

This anti-Lactoferrin antibody is a Cow Polyclonal antibody detecting Lactoferrin in WB, IP and IA. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Lactoferrin antibody (ABIN2192193)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human

Host: Cow

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunoassay (IA)

Product Details anti-Lactoferrin Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Lactoferrin antibody (ABIN2192193)

van Veen, Geerts, van Berkel, Nuijens: "Analytical cation-exchange chromatography to assess the identity, purity, and N-terminal integrity of human lactoferrin." in: Analytical biochemistry, Vol. 309, Issue 1, pp. 60-6, (2002) (PubMed).

Nibbering, Ravensbergen, Welling, van Berkel, van Berkel, Pauwels, Nuijens: "Human lactoferrin and peptides derived from its N terminus are highly effective against infections with antibiotic-resistant bacteria." in: Infection and immunity, Vol. 69, Issue 3, pp. 1469-76, (2001) (PubMed).

Nuijens, van Berkel, Geerts, Hartevelt, de Boer, van Veen, Pieper: "Characterization of recombinant human lactoferrin secreted in milk of transgenic mice." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8802-7, (1997) (PubMed).

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactoferrin

Background: Human lactoferrin (LF) is an 80 kDa glycoprotein which was first isolated from human milk. It plays an important part in the immune system and helps to fight infections. Lactoferrin promotes the health of the gastro-intestinal system by improving the intestinal microbial balance. In addition, LF can be found in epithelia and most body fluids and secretions. Lactoferrin is secreted in plasma by neutrophils. Its plasma concentration also represents a positive relation to the total pool of neutrophils and the rate of neutrophil turnover. In inflammation lactoferrin is released from secondary granules of neutrophilic leukocytes into the extracellular medium. Therefore the extracellular lactoferrin concentration can be used as an index for neutrophil activation. Lactoferrin strongly binds to iron and this iron binding property is considered to be an important antimicrobial. Human lactoferrin binds to bacterial products through its highly positively charged N- terminus, it kills various bacteria, most probably by inducing intracellular changes in these bacteria without affecting the membrane permeability. Cleavage by pepsin of lactoferrin leads to the release of lactoferricin H. This 47 amino acid peptide has more antimicrobial activity than its precursor and it can inhibit the classical but not the alternative complement pathway. Lactoferrin also plays a role in signal transduction, immunomodulation and has antiadhesive, anticancer, antiviral activity.

Pathways: Transition Metal Ion Homeostasis