Cd120b, Tnf-r2 Hp9003 antibody

Catalog No. ABIN2192231

The Rabbit Polyclonal anti- antibody has been validated for Func, IA, FACS, IP and WB. It is suitable to detect in samples from Human.

Quick Overview for Cd120b, Tnf-r2 Hp9003 antibody (ABIN2192231)

Target: Cd120b, Tnf-r2 Hp9003

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Cross reactivity: Human TNF-R1 : Yes,* Human TNF-alpha Minimal crossreactivity problematic in case of TNF-R2 knockout studies

Sterility: 0.2 μm filtered

Application Details

Application Notes: It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Antibody reactivity with cell bound or soluble TNF-R2 is not inhibited by high concentrations of human TNFα. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details

Target: Cd120b, Tnf-r2 Hp9003

Background: Type 2 (TNF-R2) of the membrane-bound as well as the soluble receptor. TNF-R2 (~75-80 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-R1 and TNF-R2, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-R1. TNF-R1 is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-R2 is efficiently activated only by the membrane bound form of TNF-alpha. Binding of the inherently trimeric TNF-alpha to TNFR1 and TNFR2 induces receptor trimerization and recruitment of several signaling proteins to the cytoplasmic domains of the receptors. Occupancy of TNFR2 results in direct recruitment of TNF Receptor Associated Factor 2 (TRAF2), which in turn recruits TRAF1. Tumor necrosis factor receptor superfamily member 1B, TNF-RII, p75/p80 Aliases Rabbit IgG