CrkL antibody (pTyr207)

Catalog No. ABIN220059

This anti-CrkL antibody is a Rabbit Polyclonal antibody detecting CrkL in WB, IHC, IHC (p), IP and FACS. Suitable for Human.

Quick Overview for CrkL antibody (pTyr207) (ABIN220059)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-CrkL Antibody

Binding Specificity: pTyr207

Specificity: Detects endogenous levels of CrkL only when phosphorylated at Tyr207. Slight cross-reactivity with Tyr221 phosphorylated CrkII. Does not cross-react with other tyrosine phosphorylated proteins. Species cross-reactivity: Human. Predicted to cross-react with mouse based on 100 % sequence homology.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr207 of human CrkL (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo (1:50), IHC, IHC-P, IP (1:50), WB (1:1000)

Usage: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:200. Flow Cytometry: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CRKL

Background: Name/Gene ID: CRKL

Synonyms: CRKL, Crk-like protein

Gene ID: 1399

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor