PKC alpha antibody (pThr638, Thr641)

Catalog No. ABIN220827

The Rabbit Polyclonal anti-PKC alpha antibody (ABIN220827) specifically detects PKC alpha in WB, ELISA and IP. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for PKC alpha antibody (pThr638, Thr641) (ABIN220827)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-PKC alpha Antibody

Binding Specificity: pThr638, Thr641

Specificity: Detects human PKC alpha only when phosphorylated at Thr638 and human PKC beta II only when phosphorylated at Thr641. Reacts weakly with phosphorylated PKC beta I and gamma. Recognizes the active form of PKC. Species cross-reactivity: Monkey and mouse. Predicted to cross-react with rat based on sequence homology.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr638 of human PKC alpha.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP (1:100), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA / PKC-Alpha

Background: Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha

Gene ID: 5578

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling