PKC alpha antibody

Catalog No. ABIN220833

This anti-PKC alpha antibody is a Rabbit Polyclonal antibody detecting PKC alpha in WB, IP, IF, FACS and ICC. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for PKC alpha antibody (ABIN220833)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-PKC alpha Antibody

Specificity: Detects endogenous levels of total PKCa protein. Does not cross-react with endogenous levels of other PKC isoform MW: 80kD. Species cross-reactivity: Monkey, Mouse and Rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH-coupled) corresponding to human PKCa.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Flo (1:25), ICC (1:50), IF (1:50), IP (1:50), WB (1:1000)

Usage: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Flow Cytometry: 1:25. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunocytochemistry: 1:50. Immunofluorescence: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA / PKC-Alpha

Background: Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha

Gene ID: 5578

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling