ENOS antibody (pSer1179)
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- Target See all ENOS (NOS3) Antibodies
- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
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Binding Specificity
- pSer1179
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Reactivity
- Human, Rat, Mouse, Pig, Cow, Dog, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ENOS antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Specificity
- Recognizes bovine eNOS protein, phosphorylated at Ser1179 (~135kD). Reactivity has been confirmed with VEGF-stimulated, serum-starved BAEC (bovine aortic endothelial cell) lysates. A very low signal was observed in the lysates of serum-starved, unstimulated BAEC and no signal was observed in lysates of unstimulated BAEC in the presence of serum. Species sequence homology: human, rat, mouse, canine, porcine, and guinea pig.
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide derived from the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product NOS3 Primary Antibody
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- Application Notes
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Approved: ELISA (0.1 - 1 μg/mL), IF, WB (1 - 3 μg/mL)
Usage: Suitable for use in Immunofluorescence, ELISA and Western Blot. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunofluorescence: reactive with monkey COS cells in immunofluorescence assays (per Fulton D, et al. 2002 publication.). - Comment
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Target Species of Antibody: Bovine
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Target
- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
- Alternative Name
- NOS3 / eNOS (NOS3 Products)
- Synonyms
- ECNOS antibody, eNOS antibody, 2310065A03Rik antibody, Nos-3 antibody, ecNOS antibody, EC-NOS antibody, NOSIII antibody, cNOS antibody, NOS antibody, NOS3 antibody, eNos antibody, ENOS antibody, nitric oxide synthase 3 antibody, nitric oxide synthase 3, endothelial cell antibody, NOS3 antibody, Nos3 antibody, nos3 antibody
- Background
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Name/Gene ID: NOS3
Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3 - Gene ID
- 4846
- UniProt
- P29474
- Pathways
- ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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