G-CSF antibody (Biotin)

Catalog No. ABIN226509

This anti-G-CSF antibody is a Rat Monoclonal antibody detecting G-CSF in WB and ELISA. Suitable for Human.

Quick Overview for G-CSF antibody (Biotin) (ABIN226509)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This G-CSF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-G-CSF Antibody (Biotin)

Specificity: Recognizes human Granulocyte-Colony Stimulating Factor (G-CSF).

Purification: Purified

Immunogen: E. coli-expressed recombinant human G-CSF.

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, WB

Usage: ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying human G-CSF protein levels.1,2 Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human G-CSF as the standard. Western Blot: Useful for Western blot applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human G-CSF under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: CSF3 / G-CSF / GCSF

Background: Name/Gene ID: CSF3

Synonyms: CSF3, C17orf33, Filgrastim, G-CSF, GCSF, Pluripoietin, CSF3OS, Lenograstim

Gene ID: 1440

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization