CTNNB1 antibody (pTyr142)

Catalog No. ABIN227283

This anti-CTNNB1 antibody is a Rabbit Polyclonal antibody detecting CTNNB1 in WB and ELISA. Suitable for Human.

Quick Overview for CTNNB1 antibody (pTyr142) (ABIN227283)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTNNB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CTNNB1 Antibody

Binding Specificity: pTyr142

Specificity: Recognizes a 92kD protein corresponding to the molecular mass of b-Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate-treated cell lines, such as A431 and human endothelial cells. Species sequence homology: mouse and rat. Sequence homology: one amino acid difference from a sequence around tyrosine 133 of human g-Catenin.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to amino acid residues around tyrosine 142 of human b-Catenin (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:2000), WB (1:500)

Usage: Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Western Blot: 1:500. Membrane was incubated with diluted antibody in 5 % non-fat milk, PBS, 0.04 % Tween 20 for 1 hour at RT. ELISA: 1:2000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: CTNNB1 / Beta Catenin

Background: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gene ID: 1499

UniProt: P35222

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2