ENOS antibody (pSer114)

Catalog No. ABIN227916

This anti-ENOS antibody is a Rabbit Polyclonal antibody detecting ENOS in WB and ELISA. Suitable for Human.

Quick Overview for ENOS antibody (pSer114) (ABIN227916)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ENOS Antibody

Binding Specificity: AA 106-118, pSer114

Specificity: Recognizes phosphorylated eNOS, Mr 132kD. Species cross-reactivity: Human and bovine. Species sequence homology: porcine.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa106-118 of human eNOS (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with porcine and bovine eNOS.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:500 - 1:1000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:500-1:1000 detected phosphorylated eNOS in fetal bovine heart endothelial cell lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3 / eNOS

Background: Name/Gene ID: NOS3

Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3

Gene ID: 4846

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling