Histone H2A antibody (acLys5)

Catalog No. ABIN228323

The Rabbit Polyclonal anti-Histone H2A antibody has been validated for WB, IHC, ELISA, IHC (p), ICC and IP. It is suitable to detect Histone H2A in samples from Human, Rat, Mouse and Monkey.

Quick Overview for Histone H2A antibody (acLys5) (ABIN228323)

Target: Histone H2A

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-Histone H2A Antibody

Binding Specificity: acLys5

Specificity: Recognizes human Acetyl-Histone H2A (Lys5) Antibody detects histone H2A only when acetylated at Lys5. This antibody does not cross-react with other acetylated histones. Species cross-reactivity: Monkey, mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic acetyl-peptide KLH coupled) corresponding to residues surrounding Lys5 of human histone H2A.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-P, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5 % BSA, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (ABC): 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Histone H2A

Target: Histone H2A