MLANA antibody

Catalog No. ABIN228816

The Mouse Monoclonal anti-MLANA antibody (ABIN228816) specifically detects MLANA in WB, FACS, IHC, IHC (p) and IP. The antibody is reactive with Human and Horse samples.

Quick Overview for MLANA antibody (ABIN228816)

Target: MLANA (Melan A (MLANA))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-MLANA Antibody

Specificity: Recognizes MART-1 at 18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not label tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Species cross-reactivity: Human and equine. Does not cross-react with mouse or rat.

Purification: Protein A purified

Immunogen: Recombinant hMART-1 protein. MW: 18kD. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. (Use Protein A). Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 1-2 μg/mL for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: CaC1 melanoma cells and melanomas.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MLANA / Melan-A / MART-1

Background: Name/Gene ID: MLANA

Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

Gene ID: 2315

UniProt: Q16655