MLANA antibody

Catalog No. ABIN228822

The Mouse Monoclonal anti-MLANA antibody (ABIN228822) specifically detects MLANA in WB, IHC, IHC (p), IP and IHC (fro). The antibody is reactive with Human and Dog samples.

Quick Overview for MLANA antibody (ABIN228822)

Target: MLANA (Melan A (MLANA))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MLANA Antibody

Specificity: Recognizes MART-1 at ~18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Does not cross-react with MAGE-1 or tyrosinase protein. Predicted species cross-reactivity: Canine.

Purification: Protein G purified

Immunogen: Recombinant MART-1 protein. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (1 - 2 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Western Blot, and Immunohistochemistry. Immunoprecipitation: Use Protein G, 2 μg antibody/mg protein lysate. Western Blot: 1 μg/mL for 2 hours at RT. Immunohistochemistry Frozen & formalin/paraffin: 1-2 μg/mL for 30 min. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: CaC1 melanoma cells and melanomas.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MLANA / Melan-A / MART-1

Background: Name/Gene ID: MLANA

Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

Gene ID: 2315

UniProt: Q16655