MLANA antibody

Catalog No. ABIN228822

This anti-MLANA antibody is a Mouse Monoclonal antibody detecting MLANA in WB, IHC, IHC (p), IP and IHC (fro). Suitable for Human and Dog.

Quick Overview for MLANA antibody (ABIN228822)

Target: MLANA (Melan A (MLANA))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MLANA Antibody

Specificity: Recognizes MART-1 at ~18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Does not cross-react with MAGE-1 or tyrosinase protein. Predicted species cross-reactivity: Canine.

Purification: Protein G purified

Immunogen: Recombinant MART-1 protein. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (1 - 2 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Western Blot, and Immunohistochemistry. Immunoprecipitation: Use Protein G, 2 μg antibody/mg protein lysate. Western Blot: 1 μg/mL for 2 hours at RT. Immunohistochemistry Frozen & formalin/paraffin: 1-2 μg/mL for 30 min. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: CaC1 melanoma cells and melanomas.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MLANA / Melan-A / MART-1

Background: Name/Gene ID: MLANA

Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

Gene ID: 2315

UniProt: Q16655