TPSAB1 antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN228830

The Mouse Monoclonal anti-TPSAB1 antibody has been validated for WB and IHC. It is suitable to detect TPSAB1 in samples from Human.

Quick Overview for TPSAB1 antibody (Alkaline Phosphatase (AP)) (ABIN228830)

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TPSAB1 antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TPSAB1 Antibody (Alkaline Phosphatase (AP))

Specificity: Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.

Purification: Ion exchange chromatography

Immunogen: Human mast cell tryptase isolated from human lung tissues.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC (0.5 - 1 μg/mL), WB

Usage: Suitable for use in Western Blot, Immunohistochemistry. Immunohistochemistry: ~0.5-1 μg/mL (1:1000-1:2000). For optimal results, tissues or cytospin preparations should be fixed in Carnoy's Fluid (60 % ethanol, 30 % chloroform, 10 % glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultra structural analysis after fixation of tissue in a modified Karnovsky's reagent (Lab Invest. (1988). 58:682-691). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 M Tris, 0.1 M sodium chloride, 0.001 M magnesiuim chloride,  pH 8.0, 15 mg/mL BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Do not freeze. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing will result in a substantial loss of activity.

Target Details for TPSAB1

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Alternative Name: TPSAB1 / Mast Cell Tryptase

Background: Name/Gene ID: TPSAB1
Subfamily: Serine S1
Family: Protease

Synonyms: TPSAB1, Alpha tryptase, Mast cell alpha II tryptase, Tryptase alpha-1, Tryptase beta I, Tryptase-III, TPSB1, Tryptase alpha II, TPS1, TPS2, Tryptase beta-1, Tryptase-1, Tryptase-I, Mast cell beta I tryptase, Tryptase alpha/beta 1, Tryptase alpha/beta-1, Tryptase beta 1, Tryptase I

Gene ID: 7177

UniProt: Q15661